Fig. 4.
Comparison of manual and automated passaging of mESCs over multiple passages. Cells processed manually at (a) a fixed 1:6 (v/v) split ratio of the previous culture and (b) a measured ICD of 20,000 cells cm−2. Cells processed by automation platform at (c) a fixed 1:6 (v/v) split ratio of the previous culture and (d) a measured ICD of 20,000 cells cm−2: (■) cell number, (•) Oct-4-GFP+, and (▴) viability. Experiments performed as described in Section 2.5. Error bars represent 1 standard deviation about the mean (n = 4).