Fig. 5.

Extracellular metabolite analysis after 2 days culture post passage of cells produced during the manual and automated cultures shown in Fig. 4: (■) l-glutamine (mmol L⁻¹), (▴) glucose (g L⁻¹), (▾) lactate (g L⁻¹), (•) ammonium (mmol L⁻¹). Cells passaged using the methods described in Fig. 4: (a) manual, fixed split (b) manual, measured ICD (c) automated, fixed split and (d) automated, measured ICD. Metabolites measured as described in Section 3.3.