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ICC analysis of embryoid bodies created after 8 passages through (i and ii) manual and (iii and iv) automated bioprocess strategies for (a) brachyury, (b) βIII-tubulin and (c) alpha-fetoprotein. Blue images (i and iii) show DAPI staining and red images (ii and iv) show actual markers. Scale bars 100 μm. ICC performed as described in Section 2.6.3.
