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. 2013 Aug 5;4:2273. doi: 10.1038/ncomms3273

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Copyright © 2013, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

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(a) FLAG-V5-expressing HEK293 cells (lane 1) or hEndoV-FLAG-V5-expressing HEK293 cells (lane 2) were established, and the hEndoV-FLAG-V5 was detected with anti-FLAG antibodies. (b) Whole cell (WCE: lane 1), cytoplasmic (Cyt: lane 2), nuclear (NE: lane 3) and cytoskeletal (lane 4) extracts were detected with anti-FLAG antibodies. The purity of these fractions was confirmed with antibodies against HSP90α, PARP1 and vimentin. (c) The expression of hEndoV in HEK293 cells was detected with anti-V5 antibodies and visualized with Alexa Fluor 568-conjugated anti-mouse IgG antibodies (red) and 4',6-diamidino-2-phenylindole dihydrochloride (blue). FLAG-V5-expressing HEK293 cells (left) and hEndoV-FLAG-V5-expressing HEK293 cells (right) are shown. Scale bars, 10 μM.