Figure 5. hEndoV is a ribonuclease specific for inosine-containing dsRNA.

(a) ³² P-labelled 20-mer dsRNA containing a 5′-IIUI-3′-specific sequence (top panel) was incubated with hEndoV (0, 1, 2 and 4 nM in each group of 4 lanes). A 20-mer ssRNA (right panel) was cleaved at the indicated positions (arrows, 11 mer). (b) ³² P-labelled 20-mer dsRNA containing a 5′-UUIU-3′-specific sequence (top panel) was incubated with hEndoV (0, 1, 2 and 4 nM in each group of 4 lanes). A 20-mer ssRNA (right panel) was cleaved at the indicated positions (arrows, 11-mer). (c) ³² P-labelled 20-mer dsRNA containing a 5′-IIUI-3′- (a: top panel, lanes 1 and 2) and 5′-UUIU-3′- (b: top panel, lanes 3 and 4) specific sequence was incubated with hEndoV (lanes 1 and 3: 0 nM; lanes 2 and 4: 4 nM). The mixtures were separated on a nondenaturing 12% polyacrylamide gel.