Figure 6. hEndoV is a structure-specific inosine-containing dsRNase.

(a) RNA substrates containing inosines within a series of mismatch regions included ssRNA (I), dsRNA (I/C) and dsRNA (I/U). Each substrate was 5′-labelled on the top strand. (b) ³²P-labelled 21-mer ssRNA or dsRNA was incubated with hEndoV (8 nM) in odd-numbered lanes. A 21-mer ssRNA (right panel) was cleaved at the indicated positions (arrows, 12-mer). (c) ³²P-labelled substrates containing inosine or deoxyinosine are indicated as ssDNA (I), ssRNA (I), ssDNA (I)+RNA (AG), ssDNA (I)+RNA (A) and ssDNA (I)+RNA (G). The underlines in ssDNA (I)+RNA (AG), ssDNA (I)+RNA (A) and ssDNA (I)+RNA (G) indicate RNA. (d) ³²P-labelled substrates shown in c were incubated with hEndoV (8 nM). Cleavage sites were analysed by electrophoresis.