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. Author manuscript; available in PMC: 2014 Aug 7.
Published in final edited form as: Neuron. 2013 Aug 7;79(3):447–460. doi: 10.1016/j.neuron.2013.05.035

Figure 7. Endogenous APP and BACE-1 in post-mortem control and AD brains.

Figure 7

(A) Frozen human control and AD brains were homogenized and fractionated (n=10 brains in each group, see Supplementary fig. 6). P100 “vesicle pellets” fractions were layered and separated on sucrose density-gradients and immuno-blotted with antibodies to APP and BACE-1. Similar to mouse brain homogenates (fig. 1E) endogenous APP and BACE-1 were largely localized to separate density-fractions. However, in AD brains, more APP was seen in the highest-density fractions that also contain BACE-1 (fractions 10 – hatched red box), suggesting APP/BACE-1 convergence.

(B) Densitometry analysis of the APP/BACE-1 fractions. For a given protein, the band-intensity in each lane was normalized to the maximum band-intensity of that protein over the 10 lanes. Note the relative increases in APP band-intensities in higher-density fractions from AD brains (red dashed box).

(C) Average APP blot-intensities plotted from the data shown in (B). Note the shift in APP distribution to higher-density fractions in AD brains. (*p < 0.05; two tailed, unpaired t-test).