Figure 3.

(a) Percent changes in fluorescence intensities of 2AP-H69 in the presence of various drug concentrations (20 mm HEPES, 70 mm NH₄Cl, 30 mm KCl, pH 7.3 at 22 °C). Fluorescence intensities at 370 nm were normalized to spectra in the absence of drug. (b) Binding curves of neomycin and paromomycin to 2AP-ΨΨΨ were fit using non-linear curve fitting to a quadratic equation.^[18] The apparent K_d was measured from three independent experiments.