Table 1.

Comparison of dissociation constants (K_ds) for H69

Ligand	Method[a]	RNA type	Kd [μM]
neomycin	Fluorescence	2AP-ψψψ	2.5 ± 0.2
	ESI-MS	2AP-ψψψ	1.6 ± 0.7
	ESI-MS	ψ ψ ψ	1.2 ± 0.4
	ESI-MS	ψm3ψψ	3.0 ± 0.6
	Fluorescence	2AP-UUU	2.0 ± 0.4
	ESI-MS	2AP-UUU	1.8 ± 0.3
	ESI-MS	UUU	1.4 ± 0.6
paromomycin	Fluorescence	2AP-ψψψ	7 ± 1
	ESI-MS	2AP-ψψψ	11 ± 3
	ESI-MS	ψ ψ ψ	10 ± 3
	ESI-MS	ψm3ψψ	11 ± 3
	Fluorescence	2AP-UUU	ND[b]
	ESI-MS	2AP-UUU	9 ± 3
	ESI-MS	UUU	8 ± 2

^[a]Experimental conditions: Fluorescence; 20 mm HEPES, 70 mm NH₄CI, 30 mm KCl, pH 7.3, ESI-MS; 150 mm NH₄OAc, pH 7.0.

^[b]ND; not determined due to too small FI changes.