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Kinase activity of TaPI4KIIγ. Autophosphorylation activity of TaPI4KIIγ detected by immunoblotting with antiphosphotyrosine (Anti-Tyr), antiphosphothreonine (Anti-Thr), and antiphosphoserine (Anti-Ser) antibodies. The molecular weights of fusion proteins TF-His-TaPI4KIIγ and TF-His are marked at the left. CBB, Coomassie brilliant blue.
