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. 2013 May 13;64(10):2859–2869. doi: 10.1093/jxb/ert129

Table 1.

Experimental details. Experiments: 1, factorial combinations of nitrogen; 2, water × N; 3, N × cultivars; 4 and 5, cultivars; and 6 cultivars × water × N. Experiments 1, 2, 3, and 6 were in large containers at Lleida and experiments 4 and 5 were under field conditions at Gimenells (NE Spain). Periodic irrigations throughout the growing season, from once a week in winter to every second day during grain filling; each microcrop was irrigated individually until water freely drained underneath the container or with sprinklers at mid-tillering (25 mm), jointing (30 mm), anthesis (60 mm), and mid-grainfilling (15 mm) under field conditions. Treatments in the containers were, watered only once at sowing to warrant germination and emergence, and when the minimal N doses was applied. Fertilization is given as decimal code 2.1 (Zadoks et al., 1974); fertilizer was applied by splitting the dose into two or three equal applications at the onset of tillering (DC 2.1), at mid-tillering (DC 2.3), and the onset of stem elongation (DC 3.1). Bold type indicate treatments within an experiment.

Growing season Experiment Experimental design Experimental approaches Sowing date and density Experimental treatments
Water regime N availability Cultivars
Soil N at sowing (kg N ha–1) Fertilization (kg N ha–1)
2006–07 1 Completed randomized design (3 replicates) Crops in large containers outdoors 24 Nov 2006; 500 plants m–2 Irrigated 70 Claudio
70 50 DC21 + 50 DC31
2007–08 2 Completed randomized design (3 replicates) Crops in large containers outdoors 14 Nov 2007; 300 plants m–2 Irrigated 30 20 DC21 Claudio
30 73.3 DC21 + 73.3 DC23 + 73.3 DC31
Rainfed 30 20 DC21 Claudio
30 73.3 DC21 + 73.3 DC23 + 73.3 DC31
2008–09 3 Completed randomized design (3 replicates) Crops in large containers outdoors 28 Nov 2006; 300 plants m–2 Irrigated 20 30 DC21 Claudio, Donduro, Simeto, Vitron
20 76.6 DC21 + 76.6 DC23 + 76.6 DC31 Claudio, Donduro, Simeto, Vitron
4 Randomized block design (3 replicates) Field 24 Nov 2008; 300 plants m–2 Rainfed 130 Claudio, Donduro, Simeto, Vitron
5 Randomized block design (3 replicates) Field 12 Dec 2008; 300 plants m–2 Irrigated 580 Claudio, Donduro, Simeto, Vitron
2009–010 6 Completed randomized design (3 replicates) Crops in large containers outdoors 26 Nov 2009; 250 plants m–2 Irrigated 20 30 DC21 Donduro, Vitron
20 76.6 DC21 + 76.6 DC23 + 76.6 DC31 Donduro, Vitron
Rainfed 30 20 DC21 Donduro, Vitron
20 76.6 DC21 + 76.6 DC23 + 76.6 DC31 Donduro, Vitron