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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: J Comp Neurol. 2012 Nov 1;520(16):3617–3632. doi: 10.1002/cne.23116

Figure 4.

Figure 4

Distribution of p-STAT3 immunoreactivity and Grp50 and 4930511J11Rik mRNA in the DMH. A,C,E: Gpr50 ISHH with p-STAT3 IHCC. B,D,F: 4930511J11Rik ISHH with p-STAT3 IHCC. mRNA hybridization is represented by silver grains, while p-STAT3 immunoreactivity is represented by brown staining. A,B: Darkfield image. C–F: Brightfield images. The DMH regions of C,D are magnified in E,F. Black arrows indicate examples of neurons doubly labeled with either Gpr50 or 4930511J11Rik riboprobe and anti-p-STAT3 antisera. Scale bars = 100 µm in B; 25 µm in F.