Table 1.
List of the used QuantiTect Primer Assays commercially available from Qiagen.
| Target | QuantiTect Primer Assay | Amplicon (bp) | T m in °C |
|---|---|---|---|
| β-actin | Rn_Actb_1_SG QuantiTect Primer Assay | 145 | 86,20 |
| Nestin | Rn_Nes_1_SG QuantiTect Primer Assay | 63 | 79,60 |
| Kruppel-like factor 4 | Rn_Klf4_1_SG QuantiTect Primer Assay | 66 | 83,05 |
| Oct4 | Rn_Pou5f1_1_SG Quantitect Primer Assay | 134 | 88,05 |
| CD9 | Rn_Cd9_2_SG QuantiTect Primer Assay | 124 | 82,20 |
| GATA-binding protein 4 | Rn_Gata4_1_SG QuantiTect Primer Assay | 70 | 81,90 |
| Aggrecan1 | Rn_Agc1_1_SG QuantiTect Primer Assay | 90 | 82,15 |
| Osteopontin | Rn_Spp1_1_SG QuantiTect Primer Assay | 122 | 83,45 |
| α-smooth muscle actin | Rn_Acta2_1_SG QuantiTect Primer Assay | 82 | 82,90 |
| PPARγ | Rn_Pparg_1_SG QuantiTect Primer Assay | 146 | 82,65 |
| Glial fibrillary acidic protein | Rn_Gfap_1_SG QuantiTect Primer Assay | 131 | 83,75 |
| Myelin basic protein | Rn_Mbp_1_SG QuantiTect Primer Assay | 117 | 83,50 |
| β3-Tubulin | Rn_Tubb3_1_SG QuantiTectPrimer Assay | 114 | 85,35 |
| Neurofilament medium | Rn_Nef3_1_SG QuantiTect Primer Assay | 112 | 82,80 |
| Integrin α 6 | Rn_Itga6_1_SG QuantiTect Primer Assay | 70 | 75,75 |
| Caspase-3 | Rn_Casp3_1_SG QuantiTect Primer Assay | 61 | 76,20 |