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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Apr 1;0:74–82. doi: 10.1016/j.jchromb.2013.02.010

Table 7.

Estimation of relative amounts of modified nucleosides in E. coli 16S RNA from data in Table 6 where molar absorptivity differences are considered. Molar absorptivities from Ref. [55].

Nucleoside Average peak
areas (Table 6)		 Molar
absorptivities
(ε × 10–3)		 Relative peak areas
based on ε
ψ 0.474  8.3 0.35
m5C 0.541  6 0.50
m7G 0.777 11 0.46
m3U 0.560  8.5 0.35
m4Cm 0.515 10 0.42
m2G 1.871 10 1.80
m62A 1.00 12 1.00