Table 1.
Vendor | Clone | Pre-treatment | Dilution | |
---|---|---|---|---|
PTEN | Cell Signaling (Beverly, MA) | D4.3 | EDTA, 45 min | 1:100 |
c-MYC | Epitomics (Burlingame, CA) | Y69 | EDTA, 45 min | 1:300 |
p27 | Transduction Lab | 57 | Citrate, 25 min | 1:4000 |
Phos-AKTa | Cell Signaling | 736E11 | EDTA, 45 min | 1:50 |
Phos-S6 b | Cell Signaling | Polyclonal | EDTA, 45 min | 1:200 |
Phos-mTOR | Cell Signaling | 49F9 | EDTA, 45 min | 1:50 |
Phosphorylation site at Ser473.
Phosphorylation site at Ser235/236.