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Harmonic content of the widefield FD-FLIM system. A) The diagram indicating the application of different offset voltages to the cathode of the intensifier. B) High harmonic contributions were obtained from the measurement of laser reflection. At 0V offset (black), 2nd to 15th harmonics were measurable but their amplitudes were not significant at harmonic > 2nd. At +1.4V offset (red), high harmonics were significant until the 8th harmonics.
