Fig. 5.
Ubi-p63E is required cell-autonomously for spermatocyte cell cycle progression and spermatid differentiation without affecting tTAF or tMAC localization. (A,B) Ubi-p63E function is required cell-autonomously in germ cells for cell cycle progression. Germline clonal analysis of (A) FRT2A controls compared with (B) magn12c. Blue columns indicate the percentage of testis with GFP-negative germ cells reaching the spermatocyte stage; red columns indicate the percentage of testis with GFP-negative germ cells reaching post-meiotic spermatid stages. Error bars indicate s.d. from three independent clone inductions. (C-D′′) Indirect immunofluorescence of arrested magn12c/23b spermatocytes stained for (C) the testis TAF fusion protein Sa-GFP, (D) the tMAC protein Topi or (C′,D′) Fibrillarin and stained with (C′,D′) DAPI. (C′′,D′′) Merge, showing Sa-GFP (green in C′′) or Topi (green in D′′), Fibrillarin (red) and DAPI (blue). Scale bars: 2 μm.