Fig. 4.
Osa regulates Dl expression at the transcriptional level in ISCs. (A-B′) The transcriptional reporter Dl-lacZ is highly expressed in ISCs in the wild-type midgut (arrows in A,A′). No, or very weak, expression of Dl-lacZ was detected in ISCs that express osaRNAi (arrows in B,B′). The wild-type control and osaRNAi were driven by esgts and stained with the antibodies indicated. (C) Analysis of the fluorescence intensity of Dl-lacZ in wild-type ISCs and ISC-like cells in the osaRNAi midgut indicates that the latter have significantly less Dl-lacZ expression. (D) Quantification of Dl mRNA in wild-type and osaRNAi midguts by qPCR. Compared with wild-type, osaRNAi midguts have significantly lower Dl mRNA levels. Data are mean ± s.e.m. Scale bars: 10 μm.