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Expression of TE-associated genes did not depend on TEAD4. (A) Six two-cell embryos (numbered 1-6) were isolated at E1.5 from Tead4-/- matings and cultured in KSOM and 5% O2 for 4 days. Each embryo produced a blastocoel (BC). (B) RNA from individual embryos was assayed by RT-PCR for the presence or absence of the indicated genes. RNA from a Tead4lox/lox blastocyst was included as a positive control (lox), and H2O was used in place of RNA to provide a negative control (not shown).
