Fig. 1.

Kinase and cyclin dependency of Ser2-P in embryonic soma and germ cells. (A) Anti-Ser2-P immunofluorescence (green) analyses of embryos exposed to RNAi targeting Cdks and cyclins, as indicated, and counterstained with DAPI (red). Arrows indicate the two primordial germ cells, Z2 and Z3 (Z2/Z3), marked by anti-PGL-1 (blue). (B) Quantification of anti-Ser2-P immunofluorescence relative to that of anti-AMA-1 (total Pol II) in either Z2, Z3 or representative somatic nuclei, normalized to vector controls. Error bars indicate s.e.m. (C) Anti-Ser2-P immunofluorescence (green) analyses of embryos exposed to RNAi targeting cdk-12 and/or fcp-1, as in A. (D) Quantification of Ser2-P immunofluorescence, as in B.