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. 2013 Sep 1;140(17):3703–3713. doi: 10.1242/dev.095778

Fig. 4.

Fig. 4.

CDK-9, CDK-12, their cyclin partners, and SPT-5 are ubiquitously expressed. (A) Expression in young adult hermaphrodites of transgenic, single-copy insertions of fluorescently tagged CDK-9 (mCherry and GFP-tagged versions show the same pattern, data not shown), cyclin T (CIT-1.2 shown, CIT-1.1 has the same pattern; patterns identical with GFP or FLAG tags, not shown), CDK-12, and cyclin K. Outlines around the adult gonad (white), oocytes (blue) and embryos (orange) show these factors are expressed in the germline and maternally loaded into embryos. Boxes outline regions expanded. (B,C) Co-staining gonads with GFP and H3K4me2 show chromosomes that lack H3K4me2 (X chromosome) also lack CDK-9 and CDK-12. Representative nuclei (dotted boxes) are shown below. (D) Expression in young adult hermaphrodite of the fluorescently tagged DSIF component SPT-5, as in A.