Figure 1.

Effect of genistein, daidzein and biochanin A on HCT-116/SW-480 human colon cancer cell proliferation. Cell survival was determined by the MTS assay and calculated as a ratio, comparing it to the vehicle-treated cells. (A–C) HCT-116 cell growth was inhibited in a time-dependent manner 24, 48 and 72 h after genistein, daidzein and biochanin A exposure. (D–F) Dose-dependent response in HCT-116 cells after compounds were treated at 24, 48 and 72 h. (G) Bar plot of HCT-116/SW-480 cells treated with genistein, daidzein or biochanin A at 48 h. HCT-116 cells showed more sensitivtity than SW-480 cells (^**p<0.01). Data are presented as mean ± SD from at least triplicate wells and 3 independent experiments.