Table S1.
Summary of discordant case analysis. Metasin vs. GeneSearch and Metasin vs. Histology—Discordance analysis.
| Case No | Metasin | GeneSearch BLNA | Histopathology | Node-based Concordance or Discordance | Case-based- Concordance/discordance | Notes | |
|---|---|---|---|---|---|---|---|
| Initial Analysis | Deeper Levels | ||||||
| 1 16837 | Negative | Macro-metastasis | Macro-metastasis | NA | Macro-metastasis Discordant | Macro-metastasis Discordant | 1 2008 case-earliest batch of cases (? RNA degradation) Macro detected in one of 2 slices given for histology in 2 levels only. Not present in third slice or in deeper level |
| 2 7587B | Negative | Negative | Macro-metastasis | NA | Macro-metastasis Discordant | Concordant |
2 Another lymph node from this case showed both histological and molecular evidence of metastatic disease-hence not a true discordant case. 3 Macro-metastasis 3 mm tumour in 1 slice |
| 3 12395 | Negative | Negative | 3Macro-metastasis 3 mm in 1 slice | NA | Macro-metastasis Discordant | Macro-metastasis Discordant | 3 Macro-metastasis 3 mm tumour in 1 slice |
| 4 12082 | Positive Micromet | Negative | Negative | Negative | Micro-metastasis Discordant | Micro-metastasis Discordant | 4 Micro met MGB 31.5, Metasin Cq for CK19 is 29.9 (Veridex cut off is 30) |
| 6 9041B | 6 Positive Micromet | 6 Positive Micromet | Negative | Negative | Micro-metastasis Discordant | Micro-metastasis Discordant | 6 9041B Cp MGB is 30.4, Metasin 29.8. Positive independent of Metasin on GeneSearch BLN Assay |
| 7 8489 | Positive Micromet | Positive Micromet | Negative | 7 ITC | Micro-metastasis Discordant | 7ITC’s present in axillary clearance | 7 ITCs seen in deeper levels and in axillary clearance specimen. Positive independent of Metasin on GeneSearch BLN Assay |
| 8 10931B | Positive Macro | Positive Micromet | Negative | Negative | Micro/Macro metastasis Discordant | Micro/Macro metastasis Discordant | 8 Positive independent of Metasin on GeneSearch BLN Assay |