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. 2013 Jun 26;14(7):13194–13212. doi: 10.3390/ijms140713194

Figure 3.

Figure 3

Reverse-phase HPLC chromatograms of the solutions (with AEMTS blocking) obtained from the long-term oxidative folding of HEL using three equivalents of DHSox at pH 8.0 for 300 min in the presence of 2 M urea. Reaction conditions were (a) [R]0 = [DHSox]0/3 = 9.1 μM at 5 °C; (b) [R]0 = [DHSox]0/3 = 9.8 μM at 15 °C; (c) [R]0 = [DHSox]0/3 = 9.0 μM at 25 °C; (d) [R]0 = [DHSox]0/3 = 9.2 μM at 35 °C; and (e) [R]0 = [DHSox]0/3 = 10.5 μM at 45 °C. See the text for details of the HPLC analysis conditions.