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. 2013 Jun 26;14(7):13194–13212. doi: 10.3390/ijms140713194

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

A reverse-phase HPLC chromatogram of the sample solution (with AEMTS blocking) obtained from the reduction pulse experiment of HEL using DTT^red as a reductant at 5 °C and pH 8.0 in the presence of 2 M urea. Folding conditions were [R]₀ = [DHS^ox]₀/3 = 9.1 μM for 240 min. The reduction pulse conditions were 6 mM DTT^red for 3 min.