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. 2013 Jun 26;14(7):13194–13212. doi: 10.3390/ijms140713194

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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HPLC chromatograms of the sample solutions (with AEMTS blocking) obtained from the oxidation pulse experiments of HEL at 5 and 35 °C and pH 8.0 in the presence of 2 M urea. Folding conditions were [R]₀ = [DHS^ox]₀/3 = 11.8 μM at 5 °C and [R]₀ = [DHS^ox]₀/3 = 10.1 μM at 35 °C for 300 min. Oxidation pulse conditions were 1.5 eq DHS^ox for 3 min at 5 °C and for 20 min at 35 °C. The reduction pulse conditions were 6 mM DTT^red for 3 min at 5 °C and 1 mM DTT^red for 2 min at 35 °C. See the text for details of the HPLC analysis conditions.