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Population changes of des[76–94], des[64–80], des[6–127], and N as a function of the reaction time of the oxidation pulse (1.5 eq DHSox) in the presence of 2 M urea at pH 8.0 at 5 and 35 °C. Folding conditions were [R]0 = [DHSox]0/3 = 10.5 μM at 5 °C and [R]0 = [DHSox]0/3 = 11.8 μM at 35 °C for 300 min. After the oxidation pulse, the same reduction pulse as Figure 8 was applied. See the text for details of the HPLC analysis conditions.
