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. 2013 Jun 26;14(7):13231–13240. doi: 10.3390/ijms140713231

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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miR-338-3p and miR-338-5p expressions. (A) miR-338-3p and miR-338-5p expression were analyzed by qRT-PCR of the total RNA extracted from AtT-20 cells. miR-338-3p levels were about twice as high as miR-338-5p levels in AtT-20 cells; (B) Schematic representation of miRNA artificial targets. Construct map of the miR-338-3p and miR-338-5p artificial targets with miR-338-3p and miR-338-5p artificial target sequences below; (C) Both miR-338-3p and miR-338-5p expression was up-regulated by each of artificial targets. However, the change of miR-338-3p was not as great as miR-338-5p (miR-338*) by their artificial target. The expression levels of miR-338-5p were significantly elevated by miR-338-5p artificial target transfection but not by transfection of the miR-338-3p artificial target or empty vector (1 and 1′. null, 2 and 2′. empty vector, 3 and 3′. miR-338-3p, 4 and 4′. miR-338-5p). Fold changes are expressed versus the empty target for each (2 or 2′). Error bars in graphs indicate standard deviations; p-values are also indicated.

miR-338-3p and miR-338-5p expressions. (A) miR-338-3p and miR-338-5p expression were analyzed by qRT-PCR of the total RNA extracted from AtT-20 cells. miR-338-3p levels were about twice as high as miR-338-5p levels in AtT-20 cells; (B) Schematic representation of miRNA artificial targets. Construct map of the miR-338-3p and miR-338-5p artificial targets with miR-338-3p and miR-338-5p artificial target sequences below; (C) Both miR-338-3p and miR-338-5p expression was up-regulated by each of artificial targets. However, the change of miR-338-3p was not as great as miR-338-5p (miR-338*) by their artificial target. The expression levels of miR-338-5p were significantly elevated by miR-338-5p artificial target transfection but not by transfection of the miR-338-3p artificial target or empty vector (1 and 1′. null, 2 and 2′. empty vector, 3 and 3′. miR-338-3p, 4 and 4′. miR-338-5p). Fold changes are expressed versus the empty target for each (2 or 2′). Error bars in graphs indicate standard deviations; p-values are also indicated.