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. 2013 Jun 26;14(7):13241–13265. doi: 10.3390/ijms140713241

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Subcellular localization of p24RFP-TMD23 and p24-TA. A, B and C: p24RFP-TMD23 was transiently co-expressed in tobacco protoplasts for 24 h with markers for the ER (GFP-HDEL), trans-Golgi cisternae (ST-YFP) or TGN/MVB (GFP-BP80). Scale bars: 10 μm (A and C); 5 μm (B). (D) Leaves of transgenic tobacco expressing p24-TA were homogenized in the absence of detergent. Subcellular compartments were separated by ultracentrifugation on isopycnic sucrose gradient. Proteins in each fraction were finally analyzed by SDS-PAGE and protein blot with antibodies against p24. Top of the gradient is at left; numbers on top indicate fraction density (g/mL). The number at left indicates the position of molecular mass marker, in kDa.