Figure 11.

In vitro release of p24 from the fusion proteins by thrombin cleavage. Leaf homogenates from transgenic tobacco expressing the indicated p24 fusions or wild type (wt) tobacco plants were immunoselected with rabbit p24 antibodies and protein A-Sepharose. The beads were then split into two equal aliquots, which were incubated with thrombin (THR+) or with buffer without enzyme as control (THR−). After centrifugation, the resin pellet (R) and supernatant (Sn) were then subjected to SDS–PAGE and protein blot using sheep anti-p24 antibodies. In each panel, the arrow at right indicates the position of p24 released by thrombin. Numbers at left indicate the position of molecular mass marker, in kDa.