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. 2013 Jun 26;14(7):13266–13281. doi: 10.3390/ijms140713266

Figure 1.

Figure 1

Figure 1

Figure 1

Characterization of NPC sphere-derived cells cultured anchorage-independently. (a) The NPC sphere-derived cells showed significantly higher colony-forming ability than the parental cells, * p < 0.01; (b) The NPC sphere-derived cells showed higher survival rates than the parental cells after cisplatin treatment; (c) The NPC sphere-derived cells showed more potent invasive behavior than the parental cells, * p < 0.01; (d) Distinct properties of the NPC sphere-derived cells and parental cells. (i) TW06 sphere cells cultured nonadherently in a serum-free medium; (ii) The differing morphologies of the TW06 parental and sphere-derived cells when cultured adherently in a 10% serum for 48 h (scale bar 100 μm); (e) Serial photographs during non-adherent culture demonstrated sphere growing progressively at 4 days (i), 8 days (ii), and 12 days (iii).