Figure 4.

DOSY-NMR measurements to NS3. The exponential decay curves of NS3 in the absence (blank squares, continuous line) and in the presence of zinc (filled squares, dotted line). The units on the y-axis are normalized intensity of the up-field shifted signals. Experiments were acquired at 25 °C, at pH 5.4 (uncorrected for isotope effects). The errors in the intensities are less than 5%, as judged from the intensity of the signal-to-noise ratio in regions of the spectra where no signals are present. The intensities for both proteins were measured by integrating in 1D-NMR experiments the most up-field shifted resonances (see Appendix).