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. 2013 Jun 27;14(7):13377–13390. doi: 10.3390/ijms140713377

Figure 2.

Figure 2

Figure 2

Labile IQGAP1 at intercellular junctions and Stable IQGAP1 in the cortex. HUVEC monolayers were permeabilized with Cytoskeleton buffer containing 0.5% Triton X-100 (CSK-Tx buffer) to remove labile proteins. (A) After CSK-Tx Buffer was added, IQGAP1 was only visualized in the cortex (Red), but not at the junctions. However, VE-cadherin was still seen at the junctions (Green); (B) After CSK-Tx Buffer was added, IQGAP1 was only visualized in the cortex (Red), and colocalizes with F-actin (Yellow).