Skip to main content
. 2013 Jun 27;14(7):13377–13390. doi: 10.3390/ijms140713377

Figure 4.

Figure 4

Expression of GFP labeled VE- α-catenin increases the electrical resistance across HUVEC monolayers. HUVECs were transfected with adenoviral vectors expressing GFP-labeled VE- α-catenin. (A) Equal amounts of protein lysates were immunoblotted for GFP or β actin. Control group was treated with PBS; (B) HUVECs were infected in six-well dishes for 48 h, and then trypsinized and seeded in the ECIS wells. Basal electrical resistance of HUVEC monolayer was increased by 64% from 10 to 70 h after infection (n = 4 for PBS treated group, n = 4 for GFP-VE- α-catenin expressing group).