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. 2013 Jun 27;14(7):13377–13390. doi: 10.3390/ijms140713377

Figure 5.

Figure 5

IQGAP1 knock-down increases basal resistance of HUVECs. HUVECs were transfected with 0.4 uM IQGAP1 siRNA or scrambled siRNA. (A) Equal amounts of protein lysate from cells transfected with scrambled siRNA and IQGAP1 siRNA were immunoblotted for IQGAP1 and β-actin; (B) The relative amounts of IQGAP1 in cells transfected with scrambled siRNA and IQGAP1 siRNA were quantified, and 75% IQGAP1 was knocked down by siRNA (n = 16); (C) HUVEC monolayers were transfected in six-well dishes for 48 h, then trypsinized and seeded in ECIS wells. Basal electrical resistance of HUVEC monolayers was continuously monitored by ECIS and resistance across cells transfected with IQGAP1 siRNA was higher than that with scrambled siRNA (n = 7). *p < 0.05 vs.scrambled siRNA at specific time points.