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. 2013 Jun 27;14(7):13377–13390. doi: 10.3390/ijms140713377

Figure 6.

Figure 6

IQGAP1 knockdown increases association of VE-cadherin with p120 and β-catenin, but decreases the association of N-cadherin with p120 and β-catenin. HUVECs were seeded on six-well dishes for 24 h and transfected with scrambled or IQGAP1 siRNA for another 48 h. HUVEC lysates were immunoprecipitated with antibodies against p120 or β-catenin, and then blotted for VE-cadherin, N-cadherin, β-catenin and α-catenin. Representative immunoblots (A,C) and quantification of three independent immunoblots after normalization to β-actin (B,D) demonstrated that reduction of IQGAP1 increased the association of p120 (B) and β-catenin (D) with VE-cadherin (VE-cad) and decreased the association of p120 (B) and β-catenin (D) with N-cadherin (N-cad). Association of p120 with β-catenin (β-cat, A,B) or β-catenin with α-catenin (α-cat, C,D) did not change. * p < 0.05 vs. respective scrambled siRNA.