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Comparison of coating, size and ζ potential values of Fe3O4@heparin obtained under different reaction conditions.
| Reaction Conditions | Starting NP (mg) | Weight increase (mg) | TB assay heparin coating (mg) | Z-A (nm) | PDI | ζ Pot (mV) |
|---|---|---|---|---|---|---|
| Water + Tween 20® | 100 | 5 | 9 | 93 | 0.25 | −46 |
| PBS | 100 | 6 | 9 | - | 0.38 | −47 |
| PBS + Tween 20® | 100 | 8 | 10 | - | 0.32 | −49 |
