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. 2013 Jun 28;14(7):13577–13591. doi: 10.3390/ijms140713577

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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ZSTK474 inhibits phosphorylation of Girdin and blocks MMP secretion in PC3 cells. (A) ZSTK474 inhibits phosphorylation of Akt and its substrate Girdin in PC3 cells. The expression levels of p-Girdin, total Girdin, p-Akt, total Akt and β-actin in the cell lysates prepared from the DMSO-treated and ZSTK474-treated PC3 cells were determined by western blot analysis. β-actin expression was determined for a quantitative control of protein amount; (B) MMP2 and MMP9 secretion into the medium following treatment of PC3 cells with the indicated concentrations of ZSTK474. Data are mean ± SD (n = 3), and are representative of two independent experiments (upper panel); Reduced proteolytic activities of MMP2 and MMP9 shown by Gelatin zymography assay (lower panel).

ZSTK474 inhibits phosphorylation of Girdin and blocks MMP secretion in PC3 cells. (A) ZSTK474 inhibits phosphorylation of Akt and its substrate Girdin in PC3 cells. The expression levels of p-Girdin, total Girdin, p-Akt, total Akt and β-actin in the cell lysates prepared from the DMSO-treated and ZSTK474-treated PC3 cells were determined by western blot analysis. β-actin expression was determined for a quantitative control of protein amount; (B) MMP2 and MMP9 secretion into the medium following treatment of PC3 cells with the indicated concentrations of ZSTK474. Data are mean ± SD (n = 3), and are representative of two independent experiments (upper panel); Reduced proteolytic activities of MMP2 and MMP9 shown by Gelatin zymography assay (lower panel).