Figure 3.

Schematic flowchart of experimental design. After Arabidopsis Col-0 seeds had grown in MS liquid medium for 7 days, seedlings were treated for 30 or 180 min with MS medium supplemented with EBR or mock solution (DMSO), followed by RNA extraction, labeling, and hybridization. Candidate miRNAs were predicted using miRU, WMD3, and psRNATarget databases. The roles of candidate miRNAs in EBR-treated seedlings were investigated by further experiments as described in the text.