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. 2013 Jul 9;14(7):14270–14286. doi: 10.3390/ijms140714270

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

EBR up-regulates miR395a in miRNA microarray analysis. (A) Hierarchical clustering of selected miRNAs expression regulated by EBR. Seedlings were treated with EBR for 30 or 180 min. miRNA expression was assessed with miRNA microarrays. Fourteen miRNAs had significantly different expression levels after EBR treatment (p < 0.05) and were further analyzed by a hierarchical clustering algorithm; and (B) Fold changes of miR395a in miRNA microarrays and qPCR analysis. miR395a was up-regulated after EBR treatment for 180 min. snoR85 was used as an internal control for normalization.