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. 2013 Jul 9;14(7):14270–14286. doi: 10.3390/ijms140714270

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

GUN5 is a target gene of miR395a. (A) The prediction was based on complementary base-pairing between miR395a and mRNA. Putative target genes for miR395a were predicted using web-based databases (miRU, psRNATarget, WMD3) and simultaneous comparisons to gene microarray data. The putative target genes of miR395a were down-regulated by EBR treatment; (B) Expression levels of GUN5 were analyzed by Q-PCR and normalized to 18S rRNA; (C) Fluorescence assay of miR395a and potential target GUN5 in the Arabidopsis PSB-D cell line. The fluorescent expression levels of GUN5 revealed significant down-regulation by miR395a; and (D) Gene expression of GUN5 measured by qPCR in miR395a knockout plants. GUN5 expression was significantly down-regulated by miR395a. *p < 0.05.