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Runx3 regulates TSP-1 expression. (a) RT-PCR analysis of TSP-1 expression in B16-F10 melanoma cells infected with empty retrovirus (control) or Runx3 bearing retrovirus with GAPDH as an internal control; (b) Western blot analysis of TSP-1 expression in Runx3 overexpressing B16-F10 melanoma cells with β-actin as an internal control; and (c) Immunofluorescence analysis of TSP-1 expression in cells lacking Runx3 (Ctrl) and cells expressing Runx3 (Runx3).
