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. 2013 Jul 11;14(7):14504–14517. doi: 10.3390/ijms140714504

Figure 1.

Figure 1

The expression and localization of osteopontin (OPN) in mouse blastocysts at the different phase of ovarian estrogen surge. (A) Real-time PCR detects the relative expression of OPN mRNA in mouse blastocysts collected from day 3 and day 4 at 08:00, 12:00 and 20:00 (*p < 0.05); (B) Representative embryonic immunofluorescence from day 3 (a–c) and day 4 at 08:00 (d–f), 12:00 (g–i) and 20:00 (j–l) by laser confocal microscopy, OPN and nuclei were stained with FITC (green) and PI (red), respectively.