Figure 2.

N-terminal domain residues important for Mms6 self-assembly. The identified Mms6 mutant proteins, all at 0.2 mg/mL, were resolved through a Superose 12 column in BC100 buffer. The molecular masses of the protein in last two peaks in panel (A) were estimated as 91 kDa and 36 kDa, whereas the last two peaks in panel (B) were estimated as 91 kDa and 20 kDa. The molecular mass of Mms6 including its His tag is 10,298 Da.