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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Tex Dent J. 2010 Jul;127(7):651–661.

Figure 3. Application of the cell-based NBC sensor system for cytological assessment of healthy and cancerous oral mucosa.

Figure 3

(a) Exfoliative cytology specimens were obtained using the OralCDx® cytobrush (http://www.sopreventable.com/How2Use.htm); (b) Next, cells were captured on the membrane filter (panel i) followed by EGFR immunolabeling (panel ii, green) and staining of the cell cytoplasm (red) and nuclei (blue) for morphometric measurement; (c) Representative images of healthy epithelia (panel i) and a cancerous lesion (panel ii) examined using the NBC sensor illustrate the increase in EGFR expression and nuclear-to-cytoplasm ratio associated with disease progression; and (d) Logistic regression and ROC analysis of individual and combined biomarkers (adapted from Weigum et al. Cancer Detect. Prevent, 2010 34).