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. 2004 Mar 1;18(5):541–558. doi: 10.1101/gad.1151204

Figure 7.

Figure 7.

Disorganized vesicular membrane structures caused by Unc51.1.KR or SynGAP expression in cultured granule neurons. (Top) Although control cells expressing VAMP2–GFP fusion protein alone displayed regularly aligned VAMP-positive vesicles along axons (A), cells overexpressing SynGAP plus VAMP2–GFP (B) or Unc51.1.KR + VAMP2–GFP (C) had irregularly positioned vesicles. Bar, 10 μm. (Middle) Line-scan was done on the neurons shown above, and average gray levels were plotted along the entire length of the axons by using Metamorph analysis software (Universal Imaging Corporation). A peak within graphs corresponds to each VAMP2–GFP-positive punctae. (Bottom) Pixel counts per each punctae were done over ∼100 punctae for each infection experiment (VAMP2–GFP, left; SynGAP + VAMP2–GFP, center; Unc51.1.KR + VAMP2–GFP, right) by using Metamorph software, and each punctae was classified according to its pixel size. The X-axis shows the range of pixel sizes, and the Y-axis is the number of punctae that falls into each pixel size category shown in the X-axis.