Fig. 2.

(a) HT1080s (green) are depicted migrating on the surface of a PEG-DA hydrogel (2D) or within a PEG-PQ hydrogel (3D) 1 day after cell seeding. Migrating cells are confined to a 100 µm wide pattern of PEG-RGDS (red) and were tracked for 6 h with the passage of time indicated by colour changes from blue to red to yellow to white. All images are representative. Scale bar = 50 µm. (b) Cell tracking data was used to calculate cell speed and cell persistence for both 2D and 3D cell migration with cell speed significantly increased with 2D migration (*p<0.05), while cell persistence trended higher with 3D migration.