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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Brain Res Mol Brain Res. 2001 Nov 1;95(0):86–95. doi: 10.1016/s0169-328x(01)00249-2

Figure 1. RT-PCR analysis of sigma receptor 1-specific mRNA transcript in mouse tissues.

Figure 1

(A) Total RNA was isolated from mouse brain (positive control), lens, RPE/choroid and neural retina. RT-PCR was carried out with primers specific for mouse sigma receptor 1 mRNA. As a negative control, the brain sample was run through the RT-PCR procedure without reverse transcriptase (- RT). (B) Restriction analysis of RT-PCR products. Shown here, the RT-PCR product from retina was gene cleaned and used for restriction analysis with PstI and StyI. (Restriction analysis for other tissues not shown). DNA standards (75 bp – 12.2 kbp) were run on the gel in parallel to determine the size of the RT-PCR products.