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Lipid-conjugated ODNs interact with TLR9 before encapsulated ODNs. Liposomes encapsulating either CpG-ODN or non-CpG-ODN [encapsulation denoted by the ODN type in parentheses in the figure caption] were made containing 0.3 mole percent PE-CpG-ODN or PE-non-CpG-ODN. BMM were treated in serum-free media with serial dilutions of liposomes for 3 h, washed and incubated in complete media for 20 h. The supernatant was collected and analyzed for secreted IL-12p40 by ELISA.
