Table 1.

Slope of each trendline showing FIQ Correction Factor/μM SNAPtide calculated for each temperature. These slopes can be used to calculate FIQ Correction Factors at SNAPtide concentrations between 0.00–10.0 μM and at reaction temperatures between 25–50°C if the 0.0 μM SNAPtide sample is assumed to have an FIQ Correction Factor of 1.00(Calculation 1). Standard deviations of the mean for the triplicate samples are shown.

Temperature	25°C	30°C	37°C	40°C	45°C	50°C
FIQ Correction Factor/μM SNAPtide Slope	−0.0264 ± 0.0013	−0.0255 ± 0.0017	−0.0247 ± 0.0007	−0.0232 ± 0.0022	−0.0255 ± 0.0015	−0.0258 ± 0.0036